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KMID : 0829320130160010039
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Korean Journal of Clinical Microbiology
2013 Volume.16 No. 1 p.39 ~ p.44
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Microbial Contamination of Donated Umbilical Cord Blood
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Park Jeong-Su
Shin Sue
Yoon Jong-Hyun
Roh Eun-Youn
Chang Ju-Young
Kim Eui-Chong
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Abstract
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Background: Testing for possible microorganism contaminationin umbilical cord blood (UCB) is essential for validating the product safety of allogeneic cellular therapeutics. We analyzed the level of contamination and related factors at the largest public cord blood bank in Korea. In addition, we also studied the influence of cryopreservation on contaminating microorganisms. Results: Among a total of 21,236 UCB, 677 (3.19%) were positive for culture. The most frequently identified organism was Lactobacillus spp. (17.2%), followedby Bacteroides spp. (10.1%), coagulase negative staphylococcus (6.4%), except the unidentified gram positive bacillus (21.4%). The contamination rate was higher in vaginal delivery specimens than in cesarean section specimens (4.1% vs. 0.7%, P＜0.001),and differed by collection center (0.7-25.4%, P＜0.001).Only 55% after-thaw cultures of non-conforming units were positive. Conclusion: We determined the contamination rate ofUCB in Korea in a large sample size. The results of this study could be used as baseline data at collectioncenters for quality control purposes. The low recovery rate of microorganisms after cryopreservation presents a possible way to rescue some non-conforming cord blood units, although further study isneeded to confirm the reduction of microbiological burden. (Ann Clin Microbiol 2013;16:39-44)
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KEYWORD
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Bacteria, Biological specimen banks, Quality control, Umbilical cord blood
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